辣根過(guò)氧化物酶作為標(biāo)記物廣泛用于各種免疫化學(xué)包括酶聯(lián)免疫反應(yīng)、免疫印跡和免疫組化中。此外，它也用于臨床檢測(cè)中過(guò)氧化氫的檢測(cè)。
Appearance: Reddish-brown amorphous powder, lyophilized
Source: Horseradish
Enzyme Commission Number: EC 1.11.1.7
CAS Number: 9003-99-0
Storage temperature: -20℃
Activity: ≥250 Purpurogallin U/mg solid
Unit definition: One purpurogallin unit causes the formation of one milligram of purpurogallin in 20s at pH 6.0 at 20 °C.
PROPERTIES
Stability: Stable at -20℃ for at least one year
Molecular weight ~40 kDa (SDS-PAGE)
Isoelectric point 7.2
Optimum pH: 6.5
Optimum temperature: 45℃
pH Stability 5.0~10.0(30℃,16hr)
Thermal stability < 50℃ (pH 6.0,15min)
Inhibitors Cyanide, sulfide, fluoride, azide